By: Dini Wahyu Yudianingtyas (08\/ 275699\/ PKH\/ 0367)<\/p>\n
Rabies disease has been spread in East Indonesia. To date only two provinces in East Indonesia are free from rabies disease (Papua Province and West Papua). Rabies conventional diagnostic\u2019s method are applied as a serial using Sellers\u2019s staining Fluorescent Antibody Test (FAT) and mouse inoculation test. Sellers\u2019s staining has some advantages and can be apllied by B and C type laboratories, particulary at endemic areas.<\/p>\n
However, Seller\u2019s staining method has been questioned by international community for its accuracy as a rabies diagnostic tool. Development of molecular based technique still on continuing process using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Aim of this study are to evaluate and describe out agreement value of Sellers staining and RT-PCR compared to FAT and optimation of one-step RT-PCR for rabies detection. Out of 130 rabies samples tested at virology and biotecnology laboratories of Disease Investigation Centre Maros. Results of Kappa showed that agreement of Sellers\u2019s staining to FAT were 0,75\u20130,80 and agreement value of one-step RT-PCR to FAT were 0,86\u20130,89 also there was no false negative result found. These results indicated high agreements from both Sellers\u2019s staining and one-step RT-PCR. These findings suggest that both techniques, Sellers\u2019s staining and one-step RT-PCR\u00a0 could be considered as tools for rabies eradication program. Further studies are required to assess the sensitivity and specificity of the rabies diagnostic tests using the gold standard.<\/p>\n
Kata kunci : virus rabies, Sellers, FAT, RT-PCR, Kappa<\/em><\/p>\n Supervisor: drh. Heru Susetya, MP., PhD and Dr. drh. Aris haryanto, MSi<\/p>\n","protected":false},"excerpt":{"rendered":" By: Dini Wahyu Yudianingtyas (08\/ 275699\/ PKH\/ 0367) Rabies disease has been spread in East Indonesia. To date only two provinces in East Indonesia are free from rabies disease (Papua Province and West Papua). Rabies conventional diagnostic\u2019s method are applied as a serial using Sellers\u2019s staining Fluorescent Antibody Test (FAT) and mouse inoculation test. Sellers\u2019s […]<\/p>\n","protected":false},"author":515,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[41],"tags":[],"class_list":["post-271","post","type-post","status-publish","format-standard","hentry","category-seminar-hasil"],"_links":{"self":[{"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=\/wp\/v2\/posts\/271","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=\/wp\/v2\/users\/515"}],"replies":[{"embeddable":true,"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=271"}],"version-history":[{"count":0,"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=\/wp\/v2\/posts\/271\/revisions"}],"wp:attachment":[{"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=271"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=271"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sainvet.fkh.ugm.ac.id\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=271"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}